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 ShameMaker
Registered: 07/13/09
Posts: 36
Loc: Rocky Mountain High State
Last seen: 7 years, 7 months
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 Has anyone tried Tissue Cloning yet? 
#318955 - 11/19/09 11:03 PM (15 years, 1 month ago) |
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One of the more recent High Times mags had an article on tissue cloning. I was wondering if anyone has tried this yet or if you know any more info on it. I will soon be running some test runs myself.
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When I feel ashamed I get so scared. It makes me want to hurt you.
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 Magash
The Feminizer
Registered: 04/21/08
Posts: 6,634
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Re: Has anyone tried Tissue Cloning yet? [Re: ShameMaker]
#318963 - 11/19/09 11:30 PM (15 years, 1 month ago) |
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It's actually pretty easy. The thing is it's slow. Now if you need huge numbers of clones from only one plant it's the way to go.
http://www.kitchenculturekit.com/Index.htm
http://www.hometissueculture.org/
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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
 Join us at the Growery!
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captain.koons
Failed Botanist
Registered: 06/25/08
Posts: 6,170
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Re: Has anyone tried Tissue Cloning yet? [Re: Magash]
#318997 - 11/20/09 12:57 AM (15 years, 1 month ago) |
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how slow is it?
I want to try this. If it takes less than 2 months I could see it being really viable for SOG growers who don't want to have a clone room.
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TROLLS NEED LOVE TOO!
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Magash
The Feminizer
Registered: 04/21/08
Posts: 6,634
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Re: Has anyone tried Tissue Cloning yet? [Re: captain.koons]
#319002 - 11/20/09 01:10 AM (15 years, 1 month ago) |
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It takes pretty close to that 2 months. Even so with a little planning you can still use it with a sog.
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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
Join us at the Growery!
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Inverted
CNC Machinist/Greenthumb
Registered: 06/01/08
Posts: 9,953
Loc: North Star 
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Re: Has anyone tried Tissue Cloning yet? [Re: Magash]
#319013 - 11/20/09 01:29 AM (15 years, 1 month ago) |
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If I had the money I'd totally buy one of those kits and give it a shot, I will need lots and lots of sprouts/small plants ready for next years outdoor season 
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Don't criticize what you can't understand
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Magash
The Feminizer
Registered: 04/21/08
Posts: 6,634
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Re: Has anyone tried Tissue Cloning yet? [Re: Inverted]
#319024 - 11/20/09 01:44 AM (15 years, 1 month ago) |
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You have plenty of time to get mother plants going.
  
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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
Join us at the Growery!
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captain.koons
Failed Botanist
Registered: 06/25/08
Posts: 6,170
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Re: Has anyone tried Tissue Cloning yet? [Re: Inverted]
#319025 - 11/20/09 01:49 AM (15 years, 1 month ago) |
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You don't need to buy a kit if you know how to work with agar/sterile mediums.
I want to look into it further. I wonder what is the determinate factor for creating one plant with a root system. I assume a single leaf could be ripped into hundreds of bits and each bit could be turned into a single plant. Making this extremely user friendly and not having like a million tiny sprouts that are unworkable close together would be key.
I imagine if leaf material was used, one could put it into a dropper or pipette and leaf material close enough together could grow into the same plant. I really don't know I'm just making hypotheses.
*Hits google*
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TROLLS NEED LOVE TOO!
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Inverted
CNC Machinist/Greenthumb
Registered: 06/01/08
Posts: 9,953
Loc: North Star
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Re: Has anyone tried Tissue Cloning yet? [Re: Magash]
#319038 - 11/20/09 03:19 AM (15 years, 1 month ago) |
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Quote:
Magash said:
You have plenty of time to get mother plants going.
That's what I like to see, at heart i'm really just an outdoor grower. I just love it so much more for some reason, mother nature takes care of her own...
I love how massive they can get outside too!
You continue to be my hero Magnum Magash!
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Don't criticize what you can't understand
Edited by Inverted (11/20/09 03:19 AM)
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Dr. Siekadellyk
Question Everything!
Registered: 04/20/09
Posts: 9,365
Loc: Ketamine
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Re: Has anyone tried Tissue Cloning yet? [Re: captain.koons]
#319098 - 11/20/09 10:13 AM (15 years, 1 month ago) |
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wow, i had no idea you could tissue clone a plant like shrooms?
thats awesome i want to try it.
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The Kratom Report...
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Triptonic
Registered: 06/13/08
Posts: 15,581
Loc:
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Re: Has anyone tried Tissue Cloning yet? [Re: Dr. Siekadellyk]
#319100 - 11/20/09 10:17 AM (15 years, 1 month ago) |
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Quote:
Dr. Siekadellyk said:
wow, i had no idea you could tissue clone a plant like shrooms?
thats awesome i want to try it.
Agreed.
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Inverted
CNC Machinist/Greenthumb
Registered: 06/01/08
Posts: 9,953
Loc: North Star
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Re: Has anyone tried Tissue Cloning yet? [Re: Triptonic]
#319112 - 11/20/09 10:40 AM (15 years, 1 month ago) |
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Yep, it's a pretty awesome deal, making thousands of potential clones from a small plant/part of the plant. I'd almost rather use this before conventional cuttings IF I had the time...
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Don't criticize what you can't understand
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ShameMaker
Registered: 07/13/09
Posts: 36
Loc: Rocky Mountain High State
Last seen: 7 years, 7 months
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Re: Has anyone tried Tissue Cloning yet? [Re: captain.koons]
#319116 - 11/20/09 11:14 AM (15 years, 1 month ago) |
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Quote:
captain.koons said:
You don't need to buy a kit if you know how to work with agar/sterile mediums.
It is possible to make thousands of clones from a single plant.
Yeah u use agar as a medium and u need a glove box (Its real similar to mushroom cloning.) there r 3 main hormones u need to add to the agar. but the trickiest part indefinitely is the sterile work that needs to be done. If u can do it right, u can have all the plants u need to last you through the year all contained in one small closet. It doesnt get any more convenient than that.
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When I feel ashamed I get so scared. It makes me want to hurt you.
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FurrowedBrow
Free yourself from yourself
Registered: 04/20/08
Posts: 12,045
Loc: Carpal Tunnel
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Re: Has anyone tried Tissue Cloning yet? [Re: ShameMaker]
#319169 - 11/20/09 01:34 PM (15 years, 1 month ago) |
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very interesting. I've got 500 petri dishes in the other room that have needed some action for a while now.
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Farmer Joe
Registered: 09/03/09
Posts: 1,707
Loc: USA
Last seen: 2 years, 1 month
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Re: Has anyone tried Tissue Cloning yet? [Re: FurrowedBrow]
#319339 - 11/20/09 07:04 PM (15 years, 1 month ago) |
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Nice link magash. I had never heard of this method of cloning until reading this post. I didn't have time to read the entire article but I plan to when I get outta work.
Hey Shamemaker:
Do you plan on documenting your tissue cloning "test run" in a grow log here at the growery?
I hope you do. I am very intrigued by this post! I can't wait to get home do more research.
 FJ
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"Marijuana may not be addictive, but growing it is" - ED Rosenthal
Maine Caregiver In 100% compliance with Maine state laws.
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Magash
The Feminizer
Registered: 04/21/08
Posts: 6,634
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Re: Has anyone tried Tissue Cloning yet? [Re: Farmer Joe]
#319387 - 11/20/09 08:34 PM (15 years, 1 month ago) |
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It really is much easier then you think. I'm telling ya anybody can do it. 
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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
Join us at the Growery!
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ShameMaker
Registered: 07/13/09
Posts: 36
Loc: Rocky Mountain High State
Last seen: 7 years, 7 months
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Re: Has anyone tried Tissue Cloning yet? [Re: Farmer Joe]
#319951 - 11/22/09 02:13 PM (15 years, 1 month ago) |
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Yeah, ill go ahead and keep you updated on my situation and post a thread in the grow journals. Keep in mind that i'm a small-time closet grower and im starting some new plants within the next week or two. So ill be trying to make a couple of clones for each plant i get going(probably 4-6). But i still need to get the proper growth hormones. Included is a pic of my homemade glove box. Sterility is the key!
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When I feel ashamed I get so scared. It makes me want to hurt you.
Edited by ShameMaker (11/22/09 02:13 PM)
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81renaissance
Coachella '13 KKOTY
Registered: 04/20/08
Posts: 4,182
Loc: State of Mind
Last seen: 10 months, 6 days
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Re: Has anyone tried Tissue Cloning yet? [Re: ShameMaker]
#319961 - 11/22/09 02:27 PM (15 years, 1 month ago) |
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 that box takes me back to the shroomie days.
Its been a minute since I cultivated fungus. 
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"So it goes."
-Kurt Vonnegut
BlueBerry_Swisher said:I want French fries. No, I want a penis French. Thank you. I'm so excited. I can not contain myself. Now I eat chocolate. It is so good. I'm trying to rub it all over myself. And then lick. Now I need a hot shower. The end.
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Farmer Joe
Registered: 09/03/09
Posts: 1,707
Loc: USA
Last seen: 2 years, 1 month
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Re: Has anyone tried Tissue Cloning yet? [Re: 81renaissance]
#320144 - 11/22/09 07:34 PM (15 years, 1 month ago) |
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nice lil lab you got there. I look forward to your log 
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"Marijuana may not be addictive, but growing it is" - ED Rosenthal
Maine Caregiver In 100% compliance with Maine state laws.
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FurrowedBrow
Free yourself from yourself
Registered: 04/20/08
Posts: 12,045
Loc: Carpal Tunnel
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Re: Has anyone tried Tissue Cloning yet? [Re: Farmer Joe]
#327395 - 12/05/09 02:15 PM (15 years, 24 days ago) |
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I just found this thread over at icmag on the subject. it's worth a read.
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