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Data said:
It looks like B. amyloliquefaciens is very similar to the model B. subtilis, so much so that it was uncertain whether they were separate species until 1987.

Here is a pdf detailing various growth media for B. subtilis culture. I'm sure any of these culture mediums would also work for B. amyloliquefaciens. Notice that at 37^oC, your population doubles every 30 minutes. If you know the bacteria density of the hydroguard, then you can determine the initial population of the LC solution, and figure out the culture time needed to reach hydroguard density. Although in all honesty, I would mix and sterilize the growth medium, inoculate and incubate at 37^o for 3-5 days to ensure all of the medium is used up, then pop in the fridge for a few days more to ensure sporulation occurs, which will allow the culture to be stored for a longer time.

Not sure if you are doing hydro or soil, but with soil, you could always mix a certain percentage of your last harvest's soil into the next crop's soil. It would effectively seed the next soil mass with the beneficial microbes from the previous batch. Might be a cheaper alternative to Liquid Cultures and whatnot.

Even with hydro, you could take a small bundle of thin rootlets from your last harvest, chum them up in a osmotically neutral solution with a blender or something, rough-filter out the bulky leftovers, and use a tiny amount of it in your res for the next crop.

Best of luck, keep us updated!

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