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Deadkndys420
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How I do it 4
#765142 - 01/12/15 01:48 PM (10 years, 1 month ago) |
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So since I got a lot of questions on how I grow my mushrooms I thought I would show you all how I do it.
Step 1. Rinsing and Soaking of grains (I'll be using WBS)
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So first you need to rinse off your grains with hot water. I use a colander in the bathtub. You'll know when the grains are ready when it runs clear.
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After that you'll need to soak the grains in water for 12-24 hrs. I also like to add a cup of brewed caffeinated coffee.
Step 2. Boiling the grains
Next you'll be boiling the grains. So pour your grains and water into your PC or large pot.
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Turn on the burner and let the water heat up. You are not trying to get a full boil going. Just hot enough that you see bubbles starting to form. You'll heat the grains for about 10-15 minutes (shorter amount of time for smaller pots). Keep an eye on the grains making sure to look for popped grains.
Next you'll rinse off the grains with hot running water. The point of this is that sometimes WBS seems to have a slimy residue left over after boiling so we need to remove this stuff.
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Step 3 Drying the grain
After you have rinse the grain you will need to dry it out. I like to dry my grains on a table with towels on the bottom and newspapers on top.
 I also have a fan blowing from the sides and a ceiling fan going on top. If you don't have a ceiling fan the side fan will work just might take a bit longer.You will 'turn' the grains about every 20 minutes. My grains are usually done drying in an hour. You'll know when the grains are dry when you pick up a handful and only a few stick to your hand.
In this picture you can see that the grains are still to wet to jar up.
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Step 4 Sterilizing
So after the grains are dry enough it's time to jar them up. You are only going to need to fill them up 3/4 of the way. And don't worry if you get some newspaper in the jar ime the mycelium will just colonize it without any adverse effect.
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Remember if you are not using Synthetic Filter Discs and/or Self Healing Injection Ports you will need to cover the jars with foil. I personally would go the SFD and SHIP route. So simple and effective.
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Okay so now that your jars are ready to sterilize put them in the PC and bring up the PSI to 15. Once you have reached 15 PSI set your timer for 90 minutes. After the jars are done let them cool for at least 10 hours (I usually do it at night so by the time I wake up they are cooled).
Step 5. Inoculation
Now that your jars are cooled its time to inoculate them with your choice of LC,spores,etc. Heat up the syringe needle until red hot. Stick the needle in the injection port (or hole) at first letting a little to cool the needle then about 1-4 ml per jar. I like using 3 ml personally. Be sure to heat up the needle red hot every time you inoculate to prevent cross contamination. I can not stress this enough. Once the jars are about 20-30 percent colonized you are gonna want to break it up by hitting the jar on a roll of duct tape being careful not to break the jar. The point of this is to redistribute the mycleium for a faster colonization rate.
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After about 3 weeks your jars should be colonized. Now its time to pasteurize the substrate.
Step 6. Pasteurization
For this you'll need 1 650 gram Coir brick 2 quarts of Vermiculite 4.5 quarts of water 1 Meat Thermometer 1 5 gal. bucket with lid 1 Towel 1 Trash Bag
1. Heat the water up in a PC or large pot to 170° 2. Toss in the coir and let it expand for 5-8 minutes 3.Toss in Vermiculite, mix very well 4.Close lid on PC or pot,put burner on high for 5 minutes, Substrate should now be 200° 5.Put the towel in the bucket followed by the trash bag, then put the substrate mix in the bucket.
 5.Twist the bag with the thermometer in the middle so heat doesn't escape. You can use a one of those big black binder clips to make sure it doesn't come undone. 6.Cover with lid and wait 75 minutes. By doing it this way you can easily keep the temp at 170°
 7.After the 75 minutes you need to let the substrate cool for a few hours.
Step 7 Spawning
Alright so now that your substrate is cool we can now spawn our colonized jars. First tape up the holes on your monotub. Next get a trashbag, cut it to size and tape it like I have done.
 Next put in 80 percent of the substrate (you need to save some for the top) in the tub and then your spawn. Mix it up so there are no big chunks of spawn or substrate. After you have mixed it well put the substrate you were saving on top being sure to cover up any exposed grains. Once you have done that remove the tape and any extra trash bag. You want the bag to stick to the substrate as it shrinks over time. This is to prevent side pins from forming.
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After that you can put the tub somewhere where it is getting ambient light. Your tub should be fully colonized in 7-10 days.
I ran into a problem where one of my tubs where fruiting before it was fully colonized. In some cases it can be a contamination and this is the myceliums last ditch effort to "spread itself via sproes". In my case it wasn't a contamination but some tape started falling off and let in Fresh Air Exchange. if you run into this fruit it and it should be okay. If you see any sign of a contamination then TOSS IT.
Step 8 Fruiting
Alright so now that your tub is colonized it's time to fruit it. Open your tub remove any excess moisture on your colonized substrate with a paper towel. By "excess moisture" I mean any standing water you see. So now that you have done that remove the tape on the holes and get out your polyfil. Refer to this LINK on how to dial in your tubs.
Now your tub is ready to go. Put a bulb (preferably 26 watt soft white CFL) on top and on a 12/12 light schedule. You will need a rotating fan on low about 6-10 feet away. If you feel the tub isn't getting enough Fresh Air Exchange then turn it on medium.
After about 2-4 days you should see some pins starting to form.
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And after about 5 or 6 days you'll see fruit bodies forming.
Step 8 Harvesting If using Multispore you will be harvesting your mushrooms at different times. Once the veil starts to open thats the time to harvest. You want to avoid the mushroom spreading its spores on the colonized substrate as this tells the mycelium to stop producing mushrooms.
So when it comes to harvesting you want to either 1.) Use scissors or garden pruners and cut at the base of the mushroom or 2.) Twist the mushroom at the base until it pulls free. The point of this is that if you just rip out clusters of mushrooms you will leave a crater. This crater can and most likely will lead to a contamination.
Step 9 Drying Well this is the moment you have been waiting for. I personally just invested in a dehydrator. You can have a ton of mushrooms dried in 12 hrs. If you don't own a dehydrator you can fan dry them. Turning them every 6 hrs or so. You can also make a desiccant and dry them that way. THIS write up tells you how to make your own desiccant from epsom salt. I have tried this and found it works pretty well.
Edited by Deadkndys420 (01/23/15 01:06 PM)
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UltimateDevotion
TwistedByDesign
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Re: How I do it (in progress) [Re: Deadkndys420] 1
#765172 - 01/12/15 05:57 PM (10 years, 1 month ago) |
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-------------------- Harry Detroit in 304 made one last promise now
"I'm goin out without a trace, a vanishing act before your eyes"
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Sham87
マリオ
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Re: How I do it (in progress) [Re: Deadkndys420] 1
#765174 - 01/12/15 06:07 PM (10 years, 1 month ago) |
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thanks for the write up.
-------------------- ...once in a while you get shown the light in the strangest of places if you look at it right...
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Ben18
Drunk Derelict
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Re: How I do it (in progress) [Re: Sham87] 1
#765237 - 01/13/15 03:50 AM (10 years, 1 month ago) |
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P-O
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Re: How I do it (in progress) [Re: Ben18] 1
#765249 - 01/13/15 09:00 AM (10 years, 1 month ago) |
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GREAT THREAD!
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Le Patron
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Re: How I do it (in progress) [Re: P-O] 1
#766880 - 01/22/15 04:56 PM (10 years, 1 month ago) |
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Yum. Tasty cubensis.
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Deadkndys420
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Done.
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Lucid
Monster Plant Creator
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Dude, nice post!
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dmtcorey
~The time police~
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Re: How I do it [Re: Lucid] 3
#769030 - 02/13/15 11:19 PM (10 years, 11 days ago) |
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--------------------
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oeric mckenna
Pure Indica
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Re: How I do it [Re: dmtcorey] 2
#769070 - 02/14/15 04:27 PM (10 years, 10 days ago) |
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What a wild veil on that beast
-------------------- ***Handing someone your life's work....in a single seed = ***
HIGHER THAN A GEORGIA PINE
Oeric's Winter Grow Projects
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dmtcorey
~The time police~
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ya its a nice tear ,little passed due tho but soon enough iim going to build a flow hood so I can isolate strains so I can have trays that are ready to harvest all at the same time instead of some being ready hear and there
I see dk420 has something isolated there with a nice even harvest time, nice work btw
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Edited by dmtcorey (02/14/15 11:25 PM)
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Deadkndys420
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Re: How I do it [Re: dmtcorey] 1
#769433 - 02/19/15 01:30 PM (10 years, 5 days ago) |
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Haha nah its not a isolate. I just got lucky with this multispore.
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dmtcorey
~The time police~
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shit eh lol , im just doing a multispore then cloning fruits in hopes I can narrow things down to the desired fruits im looking for .
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Re: How I do it [Re: dmtcorey] 1
#770154 - 02/25/15 12:19 PM (9 years, 11 months ago) |
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Some dank boomers no doubt
-------------------- Officially a Colorado medical marihuana patient
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newbie2shoes
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Im due to fruit my first jar maybe today. Looking to switch to grain for g2g or wbs after I know my sterilization technique on point. My bioluminescent jars are glowing real nice!
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iL0g1cal
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Great info.
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cheezymold
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Re: How I do it [Re: iL0g1cal] 1
#777480 - 05/01/15 01:25 AM (9 years, 9 months ago) |
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Good stuff. Ever tried growing sclerotia? I hear it is easier to stomach and doesn't taste like nasty ass cubes lol.
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H J Farnsworth
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Re: How I do it [Re: cheezymold] 1
#777646 - 05/03/15 06:02 AM (9 years, 9 months ago) |
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I like the taste of nasty ass cubes
-------------------- Officially a Colorado medical marihuana patient
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Ghost0420
Illicit
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Great writeup What's the fluffy stuff that fills the holes in monotubs?
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Deadkndys420
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Re: How I do it [Re: Ghost0420] 1
#797760 - 10/31/15 06:41 PM (9 years, 3 months ago) |
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Polyfil aka pillow stuffing.
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Ghost0420
Illicit
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How many polyfill holes do you put in the monotub and how big are the holes?
How do you cut the holes out of the monotub and where are the holes placed on the monotub?
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Deadkndys420
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Re: How I do it [Re: Ghost0420]
#797766 - 10/31/15 07:25 PM (9 years, 3 months ago) |
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You use as much polyfil as you need there's no set amount. But you want the holes on top looser then the bottom ones. The holes are two inches in diameter. The easiest way to cut the hole is using a hole saw drill bit. They are placed about 6 inches high from the bottom of the tub. And about 4 inches low from the top of the tub.
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Ghost0420
Illicit
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Thanks friend 
How many holes in total?
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Krevgin
Farmer
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I'm sorry about resurrecting this thread, but I wanted to ask about something you said. You mentioned that the mycelium would stop producing mushrooms if it contacted spores from the fresh shrooms. I just wanted to know your source for this. I haven't ever heard anything like that before, and it seemed like an interesting fact. I know if the little guys are allowed to drop spores they lose potency, but spores halting fruiting is new to me.
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Deadkndys420
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Re: How I do it [Re: Krevgin]
#801084 - 12/11/15 05:19 PM (9 years, 2 months ago) |
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Its pretty common knowledge that spores dropping on mycelium will "tell" the mycelium to stop producing fruit bodies. I think I saw it from rogerrabbit or another mod on the mushroom cultivation fourm on the shroomery. This was a few years ago.
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Dontworry
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What is wrong with side pins?
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Dr. Siekadellyk
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Hey bud how coarse is the verm u get for your substrate?
N u think a few big holes with polyfill will do better than a shotgun terrarium?
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Deadkndys420
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The verm I use is very fine so I actually double it up (2 quarts of verm).
And if you're doing a monotub type grow (using spawn and substrate) then the 6 two inch holes would probably do better. If you used a shotgun FC then I would think it would dry out a lot quicker. Also you're more susceptible to get a contamination considering the holes have no filter I.e. polyfil.
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Bobabouy
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Shotgun fruiting chambers are not suitable for bulk grows. PF cakes only!
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Dr. Siekadellyk
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No casing?
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Dr. Siekadellyk
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I've always done cakes or bags but im ready to start bulk, I aint fuckin around no more. I'm sick of being broke n having nothing so Im goin out n buying a new bigger PC today to get started.
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Deadkndys420
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Quote:
Dr. Siekadellyk said: No casing?
Nah I don't. Everything I read shows cubes don't benefit from a casing layer.
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Deadkndys420
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BTW try to do a log if you can.
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Dr. Siekadellyk
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What size tub do u use for this tek bro?
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Deadkndys420
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I believe my totes are 18 gallon. The sticker came off a few years ago.
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What type of courseness do u use of the vermiculite?
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Deadkndys420
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I use fine (all i can get atm) and double the amount I'm suppose to use. Seems to work out well.
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How many jars u use usually per one of these tubs?
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Dr. Siekadellyk
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Also is it OK if some of the seeds popped or are we Reyna cut down on that?
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Deadkndys420
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I use between 6-8 quarts per tub. And nah I wouldn't think it would matter if some seeds germ. But I never ran into that specific problem.
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Dr. Siekadellyk
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By popped I meant cooked to the point of a few splitting open. Also how much water do u normally use in your cooker for this cycle? Cuz I got moisture under my foil, is that normal?
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Deadkndys420
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A few popped grains would not matter. But if there's a lot it could lose a problem.
I use about an inch or so of water. And some moisture is to be expected. But if you see pools of water I would be concerned.
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Dr. Siekadellyk
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Just got the first sign of growth in one jar. Day 6 from innoc.
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Deadkndys420
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So I got 3 jars going strong but the others Nada. Say I get only 3 should I cut the substrate recipe I spawn to or just use the 3 following the same tek?
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Deadkndys420
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I would cut it down. Or if you want you can PC some more jars and do grain to grain transfer with those jars that have growth.
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Dr. Siekadellyk
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How come u would cut itbdo2n instead of just spawning the 3?
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Deadkndys420
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Bump
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