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Mahavatar said:
So, I have a box full of seedlings between 1 and 5 inches tall. Some are in only about a half an inch of soil, some others in about 2 inches of soil and a couple more in 2 inches of vermiculite.

The ones in the half inch look great nice and strong, bigger leaves than the others, only about 2.5inches tall though, the ones in verm look good too but nothing special.

But for some reason the ones in 2 inches of dirt are all about 2 inches taller than the rest with thin stems and cant hold themselves without help, whats the problem here?

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What kind of light are you using, how close is it to the seedlings, and how are the different mediums arranged around it? The way the ones in two inch soil have grown really sounds like they are not getting adequate light and are stretching like crazy to find some because it is a matter of survival. Otherwise I don't see it having anything to do with the type of soil or how much there was, but I'm not the most informed on this kind of stuff, so hopefully someone else will comment.

The only other factor that I can personally think of is maybe the ones in a half inch of soil fully rooted in that much soil and began focusing more on vegetative growth instead of root production, but I don't think that really makes that much sense and doesn't explain the stretching of the two inch soil seedlings.

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Also when should I transplant these guys into some styrofoam cups and when I do should I use organic soil, verm or a mix of both?

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I think the best time to transplant at any time is when the roots have fully grown through the amount of soil they are currently in and maybe begin to circle around the edge a little bit, so they are completely ready to explode with new growth when dropped into more new soil.

I don't know too much about soil mixes yet, but a mixture of both would probably be great. Better wait for someone else to come along to answer that. I'll read more into it though.