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 petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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 micro propagation media formula 
#350662 - 01/20/10 08:54 AM (14 years, 9 months ago) |
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Does anyone know where I can go to find MJ specific formulas for micro propagation media? It seems that the exact recipe for your plant of choice is necessary for any hope of success.
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 DrGreenThumb
 
Registered: 08/09/08
Posts: 487
Loc: Phish Tour 
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Re: micro propagation media formula [Re: petridish]
#350663 - 01/20/10 09:03 AM (14 years, 9 months ago) |
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If that's the case then you might want to start with soil. 
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“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.” -Dr. Seuss
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: DrGreenThumb]
#350664 - 01/20/10 09:36 AM (14 years, 9 months ago) |
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Thanks for the advice, do you know anything about media formulas?
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DrGreenThumb
Registered: 08/09/08
Posts: 487
Loc: Phish Tour
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Re: micro propagation media formula [Re: petridish]
#350667 - 01/20/10 09:59 AM (14 years, 9 months ago) |
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Not really. If you have the thousands of dollars and access to resources it would take to set of a lab to properly micropropagate, why wouldn't you just buy a few 1000W HPS's and set up a grow room.
Anyone with the knowledge to micropropagate would NOT have to come to the Growery to find proper media formulations. The reason marijuana growers do not micropropagate is because it's very expensive, prone to failure and IMPRACTICAL.
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“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.” -Dr. Seuss
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: DrGreenThumb]
#350671 - 01/20/10 10:19 AM (14 years, 9 months ago) |
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I'm not looking to set up a big ass grow show. I have the equipment and facilities I need and certainly did not invest that heavily. I am very familiar with tissue culture and am not asking if it is easy or practical. If you don't have any idea as to how to answer my simple question, why don't you refrain from replying?
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DrGreenThumb
Registered: 08/09/08
Posts: 487
Loc: Phish Tour
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Re: micro propagation media formula [Re: petridish]
#350673 - 01/20/10 10:24 AM (14 years, 9 months ago) |
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Ok, Chill. Don't get your panties in a bunch. Personally, if I had access to that type of equipment and facilities I'd be doing something worth while; like making ecstasy! Good luck finding your answers.
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“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.” -Dr. Seuss
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: petridish]
#350675 - 01/20/10 10:35 AM (14 years, 9 months ago) |
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I don't believe x or any other forms of meth are worth while. I doubt my lab interests will ever go beyond tissue culture. I would love to hear from anyone with any direction to go looking in for formulas!
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: petridish]
#350684 - 01/20/10 11:01 AM (14 years, 9 months ago) |
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How about Hops(humulus lupulus), a close relative?
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DrGreenThumb
Registered: 08/09/08
Posts: 487
Loc: Phish Tour
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Re: micro propagation media formula [Re: petridish]
#350688 - 01/20/10 11:06 AM (14 years, 9 months ago) |
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Didn't mean to come off like an ass; you got me researching though. Check this link: http://www.pakbs.org/pjbot/PDFs/41%282%29/PJB41%282%29603.pdf That's the closest I could find. Formula is right there.
I'm not saying it can't be done. What I'm saying is that people that GROW herb don't micropropagate. For reasons I mentioned earlier.
If you're only looking to grow a little smoke for yourself and friends go buy a 400W HPS and some seeds. I'm not telling you not to do it. I'm just trying to be helpful (and practical).
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“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.” -Dr. Seuss
Edited by DrGreenThumb (01/20/10 11:07 AM)
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: petridish]
#350719 - 01/20/10 12:10 PM (14 years, 9 months ago) |
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Hemp, well no screamin eagle shit! Didn't even dawn on me to check that out. That link is perfect. Without those formulas it wouldn't have been feasible for me to play with this. I don't have the money or time to start from scratch and try it all. I'm not thinking this is some great easy way to grow herb. Sometimes I do things because I can or to find out if I can. Something sparked this interest as a way around a very special problem. If it proves to be a useful way around said problem I will share. I'm hopeful at best. I look forward to working with plant tissue even if it never provides anyone with any smoke Thanks for not just pointing me in the right direction, but for giving me exactly what I needed!
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: petridish]
#350720 - 01/20/10 12:13 PM (14 years, 9 months ago) |
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where did you find that?
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DrGreenThumb
Registered: 08/09/08
Posts: 487
Loc: Phish Tour
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Re: micro propagation media formula [Re: petridish]
#350722 - 01/20/10 12:23 PM (14 years, 9 months ago) |
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No problem, glad I could help out. Welcome to the Growery by the way. Didn't mean to be a stick in the mud earlier. Stick around here a while and you might just learn something. Maybe you can teach us a thing or two. Knowledge is power. 
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“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.” -Dr. Seuss
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DrGreenThumb
Registered: 08/09/08
Posts: 487
Loc: Phish Tour
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Re: micro propagation media formula [Re: DrGreenThumb]
#350724 - 01/20/10 12:25 PM (14 years, 9 months ago) |
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It's one of the first results in Google. Just type "micropropagation cannabis". 
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“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.” -Dr. Seuss
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mhbound
Ballin out at all cost
Registered: 09/22/08
Posts: 8,144
Loc: High
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Re: micro propagation media formula [Re: DrGreenThumb]
#350788 - 01/20/10 02:52 PM (14 years, 9 months ago) |
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fuck google
i like to BING
no fuck bing and microsoft
and george bush jr and sr
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Suck my balls America
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canndo
Stranger
Registered: 03/23/11
Posts: 7
Last seen: 13 years, 7 months
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Re: micro propagation media formula [Re: petridish]
#539264 - 03/23/11 02:46 PM (13 years, 7 months ago) |
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Quote:
petridish said:
I don't believe x or any other forms of meth are worth while. I doubt my lab interests will ever go beyond tissue culture. I would love to hear from anyone with any direction to go looking in for formulas!
I have perfected several protocols and am considering selling the chemicals as pre made formulas. I have establishment, multiplication, callus, seed starting, and one for dirty plants.
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canndo
Stranger
Registered: 03/23/11
Posts: 7
Last seen: 13 years, 7 months
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Re: micro propagation media formula [Re: canndo]
#539266 - 03/23/11 02:49 PM (13 years, 7 months ago) |
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BTW, that Chinese protocol isn't bad but it gives you lots of chances for vitrification, something you don't want. Look into GA3.
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: canndo]
#610716 - 02/26/12 08:25 PM (12 years, 8 months ago) |
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Cool, thanks. I like the avatar and hope to hear more from you
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aris
Stranger
Registered: 02/27/12
Posts: 3
Last seen: 11 years, 4 months
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Re: micro propagation media formula [Re: petridish]
#610736 - 02/27/12 10:26 AM (12 years, 8 months ago) |
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necro thread lives again....
there are pdf files of "plants from test tubes" floating around the net. i dont think this stuff is too expensive or rocket science either. i have seen mediums that were food safe like coco water and banana goo. Try search "kitchen chemistry" tissue culture. Usenet news feeds actually have a whole lot of active tissue culture and meristem groups.
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: aris]
#610894 - 02/28/12 02:34 PM (12 years, 8 months ago) |
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Any forums similar to this or the Shroomery?
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kyuzo
Stranger Than Fiction
Registered: 07/05/10
Posts: 981
Last seen: 11 years, 10 months
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Re: micro propagation media formula [Re: petridish]
#610903 - 02/28/12 03:07 PM (12 years, 8 months ago) |
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there is another micro prop thread here with some good resources on small amounts of hormones from plant societies that i posted, and what not
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Stoneth
D.I.L.L.I.G.A.F.
Registered: 10/06/08
Posts: 25,048
Loc: No where ville, USA
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Re: micro propagation media formula [Re: kyuzo]
#610905 - 02/28/12 03:18 PM (12 years, 8 months ago) |
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http://www.growery.org/forums/showflat.php/Number/534818#534818
There's a bit of info on the subject.
I know there's another good thread around here some where on it as well.
I'll keep looking.
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petridish
Stranger
Registered: 01/15/10
Posts: 15
Last seen: 11 years, 5 months
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Re: micro propagation media formula [Re: kyuzo]
#611244 - 03/02/12 10:26 AM (12 years, 8 months ago) |
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I read those, thanks. I'm hoping someone has recipes for different media specifically designed for MJ. I feel safer asking here. I wouldn't ask just anybody. Some folks get all uptight.
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Microclone
Stranger
Registered: 05/14/17
Posts: 3
Last seen: 7 years, 6 months
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 Re: micro propagation media formula [Re: petridish]
#825905 - 05/15/17 12:55 AM (7 years, 6 months ago) |
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Edited by Data (05/15/17 04:52 PM)
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Data
That Guy
Registered: 08/12/08
Posts: 4,035
Loc: Southwestern US
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Re: micro propagation media formula [Re: Microclone]
#825914 - 05/15/17 04:59 PM (7 years, 6 months ago) |
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Microclone, the last post of this thread was made 5 years ago, and the user petridish hasn't been around for almost 4 years.
In the future, you can find this info in the subject line in the last post, and the information of the user to the left of each post, respectively.
Obviously, feel free to respond to older threads if you feel the information is relevant, just make sure to follow the rules of the board when posting.
Thanks, and welcome 
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“The Universe is under no obligation to make sense to you” -NDT
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Theman
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Registered: 10/06/16
Posts: 344
Last seen: 4 years, 4 months
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Re: micro propagation media formula [Re: Data]
#825915 - 05/16/17 12:13 PM (7 years, 5 months ago) |
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Hmm can we see picks of liquid culture? Do they grow much more then just rooting the cuttings?
Sorry i just saw video ty
What if we just used the nutrient hormome lc solution in say rockwool or perlite and planted microclo es directly into that in a domed plant tray? Then replace solution with rooting harmone solution later on when saw leaves? I know small advantage but save a step of bottling. Perhaps using peroxide to mantain relative cleanleness?
Suppose only bottling when wanting to store microcloned mother??
Edited by Theman (05/16/17 12:34 PM)
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We have made it so easy to get plants into culture and back out again. The time in between is a matter of successive cultures to store the mother tissue. You can stretch the storage time by chilling the cultures to 58 degrees but the best new technique we are using from the kit is putting two inch cuttings into liquid culture for two weeks and them planting them in root plugs. 
